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=DNA quantification= | =DNA quantification= | ||
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| + | ==GeneQuant/Genequant II== | ||
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| + | GeneQuant Manual: http://www.americaninstrument.com/pdf/1976E-DNA.pdf | ||
==Eppendorf biophotometer 6131== | ==Eppendorf biophotometer 6131== | ||
Revisjonen fra 6. aug. 2016 kl. 23:55
An overview of available equipment and equipment of interest.
Innhold
DNA quantification
GeneQuant/Genequant II
GeneQuant Manual: http://www.americaninstrument.com/pdf/1976E-DNA.pdf
Eppendorf biophotometer 6131
Manual: http://arboretum.harvard.edu/wp-content/uploads/Biophotometer-manual.pdf
- Device: Single-beam filter photometer with reference beam and fixed wavelengths.
- Light path:
Cuvette types (min volume):
- 10 mm macro (1000 uL)
- 10 mm semi-micro (400 uL)
- 10 mm suction (300 uL)
- 10 mm ultra-micro (70 uL)
Wavelengths: 230,260,280,320,562,595
Height of light beams in cuvette: 8.5 mm
Kompatible kuvetter:
https://www.brandtech.com/product/uv-cuvettes/
Centrifugation
Eppendorf 5415D
Manual: http://www.nist.gov/ncnr/upload/Eppendorf_5415R_Centrifuge_Manual.pdf
- Max speed: 13 200 rpm
- Max centrifugal force: 16 110 rcf
- Max load: 24 x 2,0 mL or 36 x 0,5 mL tubes (rotor dependent)
- Power requirement: 180 W
- Weight without rotor: 8.5 kg
