Fil:July11PCRresult.jpg

The result after electrophoresis of the PCR samples from the meetup. Unfortunately, the results were rather disappointing. Out of the six PCR reactions, it appears that only one resulted in succesful amplification. Sample 5, using 1 uL of the solution previously prepared from dry baker's yeast as template, gave the expected band. There are several possible explanations for the failure of the other reactions to produce visible bands, including differences in template preparation, amount of template used and genetic differences between the yeast types. From left to right: DSbio 1kb ladder, PCR samples #1 , 2, 3, 4, 5, 6 and 7 (negative control). Electrophoresis was performed with 1% agarose at 100 V for 30 min.

Samples:

1. 1/HS1A: 1 uL yeast sample #1. (S. Cerevisiae [1])
2. 2/HS1B: 2 uL yeast sample #1. (S. Cerevisiae [1])
3. 3/HS2A: 1 uL yeast sample #2. (suspected Brettanomyces [1])
4. 4/HS2B: 2 uL yeast sample #2. (suspected Brettanomyces[1])
5. 5/P1: 1 uL S. cerevisiae (Idun tørrgjær. Template prepared 5 july stored in freezer)
6. 6/P2: 2 uL S. cerevisiae (Idun tørrgjær. Template prepared 5 july stored in freezer)
7. 7/NT: Reaction mix and primers only, no template.

[1]: Liquid cultures provided by Heikki. To prepare template, 50 uL liquid culture was incubated at 98C for 10 min in OpenPCR.