Fil:ITS1 ITS4 S288C BLAST result.png

Graphic representation of result returned from BLAST search showing the location of the PCR target regions for the ITS1 and ITS4 primers in the S. cerevisae S288C genome (assembly R64, GenBank accession GCA_000146045.2). The query sequence is the expected PCR product sequence returned by the UCSC in silico PCR tool. While there are two target regions, there is only one expected product sequence as the sequence at and between the primer binding sites is identical for the two target regions.

The locations of the target regions are shown as grey bands along the bottom, with the text "Query_185011" above each band. The left-pointing arrowing on each band indicates that the sequence of the query matches the sequence of the reverse strand as defined in the genome sequence file.