BioHackerLab/Equipment

An overview of available equipment and equipment of interest.

Thermocyclers

http://www.the-scientist.com/?articles.view/articleNo/18812/title/How-Low-Can-You-Go---Nineteen-Thermal-Cyclers-Priced-Under--5000/

DNA quantification

GeneQuant/Genequant II

GeneQuant Manual: http://www.americaninstrument.com/pdf/1976E-DNA.pdf

• Light path height = 15 mm

Eppendorf biophotometer 6131

Manual: http://arboretum.harvard.edu/wp-content/uploads/Biophotometer-manual.pdf

• Device: Single-beam filter photometer with reference beam and fixed wavelengths.
• Light path:

Cuvette types (min volume):

• 10 mm macro (1000 uL)
• 10 mm semi-micro (400 uL)
• 10 mm suction (300 uL)
• 10 mm ultra-micro (70 uL)

Wavelengths: 230,260,280,320,562,595

Height of light beams in cuvette: 8.5 mm

Kompatible kuvetter:

https://www.brandtech.com/product/uv-cuvettes/

See also: http://www.biocompare.com/Application-Notes/138277-Reproducible-photometric-determination-of-DNA-concentrations-using-the-Eppendorf-UVette-in-the-Eppendorf-BioPhotometer-plus/

Centrifugation

Eppendorf 5415D

Manual: http://www.nist.gov/ncnr/upload/Eppendorf_5415R_Centrifuge_Manual.pdf

• Max speed: 13 200 rpm
• Max centrifugal force: 16 110 rcf
• Max load: 24 x 2,0 mL or 36 x 0,5 mL tubes (rotor dependent)
• Power requirement: 180 W
• Weight without rotor: 8.5 kg