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(→Eppendorf biophotometer 6131) |
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An overview of available equipment and equipment of interest. | An overview of available equipment and equipment of interest. | ||
| − | =Eppendorf biophotometer 6131= | + | =DNA quantification= |
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| + | ==Eppendorf biophotometer 6131== | ||
Manual: http://arboretum.harvard.edu/wp-content/uploads/Biophotometer-manual.pdf | Manual: http://arboretum.harvard.edu/wp-content/uploads/Biophotometer-manual.pdf | ||
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https://www.brandtech.com/product/uv-cuvettes/ | https://www.brandtech.com/product/uv-cuvettes/ | ||
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| + | =Centrifugation= | ||
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| + | ==Eppendorf 5415D== | ||
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| + | Manual: http://www.nist.gov/ncnr/upload/Eppendorf_5415R_Centrifuge_Manual.pdf | ||
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| + | Max speed: 13 200 rpm | ||
| + | Max centrifugal force: 16 110 rcf | ||
| + | Max load: 24 x 2,0 mL or 36 x 0,5 mL tubes (rotor dependent) | ||
| + | Power requirement: 180 W | ||
Revisjonen fra 5. aug. 2016 kl. 21:24
An overview of available equipment and equipment of interest.
DNA quantification
Eppendorf biophotometer 6131
Manual: http://arboretum.harvard.edu/wp-content/uploads/Biophotometer-manual.pdf
- Device: Single-beam filter photometer with reference beam and fixed wavelengths.
- Light path:
Cuvette types (min volume):
- 10 mm macro (1000 uL)
- 10 mm semi-micro (400 uL)
- 10 mm suction (300 uL)
- 10 mm ultra-micro (70 uL)
Wavelengths: 230,260,280,320,562,595
Height of light beams in cuvette: 8.5 mm
Kompatible kuvetter:
https://www.brandtech.com/product/uv-cuvettes/
Centrifugation
Eppendorf 5415D
Manual: http://www.nist.gov/ncnr/upload/Eppendorf_5415R_Centrifuge_Manual.pdf
Max speed: 13 200 rpm Max centrifugal force: 16 110 rcf Max load: 24 x 2,0 mL or 36 x 0,5 mL tubes (rotor dependent) Power requirement: 180 W
