Forskjell mellom versjoner av «BioHackerLab/Experimental»
(→11 Jul 2016 - Bitraf PCR #3) |
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[1]: Liquid cultures provided by Heikki. To prepare template, 50 uL liquid culture was incubated at 98C for 10 min in OpenPCR. | [1]: Liquid cultures provided by Heikki. To prepare template, 50 uL liquid culture was incubated at 98C for 10 min in OpenPCR. | ||
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+ | ==Expected results/bioinformatics analysis== | ||
+ | |||
+ | '''Reference sequence:''' | ||
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+ | One of the yeast samples was described as Brettanomyces. The most relevant organism in this genus appears to be Brettanomyces bruxellensis. The organism overview page at http://www.ncbi.nlm.nih.gov/genome/11901 lists Brettanomyces bruxellensis CBS 2499 (assembly Dekkera bruxellensis CBS 2499 v2.0, Assembly accession no. GCA_000340765.1) as the representative genome for this organism. | ||
+ | |||
+ | '''BLAST search:''' |
Revisjonen fra 19. jul. 2016 kl. 20:12
Experiments performed at Bitraf.
Innhold
05 Jul 2016 - Bitraf PCR #1
10 Jul 2016 - Bitraf PCR #2
11 Jul 2016 - Bitraf PCR #3
PCR was performed using the same PCR setup and program as July 10 except for the selection of templates and template amounts. The following templates and template amounts were used:
- #1/HS1A: 1 uL yeast sample #1. (S. Cerevisiae [1])
- #2/HS1B: 2 uL yeast sample #1. (S. Cerevisiae [1])
- #3/HS2A: 1 uL yeast sample #2. (Brettanomyces? [1])
- #4/HS2B: 2 uL yeast sample #2. (Brettanomyces?[1])
- #5/P1: 1 uL S. cerevisiae (Idun tørrgjær. Template prepared 5 july stored in freezer)
- #6/P2: 2 uL S. cerevisiae (Idun tørrgjær. Template prepared 5 july stored in freezer)
- #7/NT: Reaction mix and primers only, no template.
The PCR samples were moved to freezer after completion of the PCR run.
[1]: Liquid cultures provided by Heikki. To prepare template, 50 uL liquid culture was incubated at 98C for 10 min in OpenPCR.
Expected results/bioinformatics analysis
Reference sequence:
One of the yeast samples was described as Brettanomyces. The most relevant organism in this genus appears to be Brettanomyces bruxellensis. The organism overview page at http://www.ncbi.nlm.nih.gov/genome/11901 lists Brettanomyces bruxellensis CBS 2499 (assembly Dekkera bruxellensis CBS 2499 v2.0, Assembly accession no. GCA_000340765.1) as the representative genome for this organism.
BLAST search: