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* #1/HS1A: 1 uL yeast sample #1. (S. Cerevisiae [1]) | * #1/HS1A: 1 uL yeast sample #1. (S. Cerevisiae [1]) | ||
* #2/HS1B: 2 uL yeast sample #1. (S. Cerevisiae [1]) | * #2/HS1B: 2 uL yeast sample #1. (S. Cerevisiae [1]) | ||
− | * #3/HS2A: 1 uL yeast sample #2. (Brettanomyces [1] | + | * #3/HS2A: 1 uL yeast sample #2. (Brettanomyces? [1]) |
− | * #4/HS2B: 2 uL yeast sample #2. (Brettanomyces [1] | + | * #4/HS2B: 2 uL yeast sample #2. (Brettanomyces?[1]) |
* #5/P1: 1 uL S. cerevisiae (Idun tørrgjær. Template prepared 5 july stored in freezer) | * #5/P1: 1 uL S. cerevisiae (Idun tørrgjær. Template prepared 5 july stored in freezer) | ||
* #6/P2: 2 uL S. cerevisiae (Idun tørrgjær. Template prepared 5 july stored in freezer) | * #6/P2: 2 uL S. cerevisiae (Idun tørrgjær. Template prepared 5 july stored in freezer) |
Revisjonen fra 19. jul. 2016 kl. 19:23
Experiments performed at Bitraf.
05 Jul 2016 - Bitraf PCR #1
10 Jul 2016 - Bitraf PCR #2
11 Jul 2016 - Bitraf PCR #3
PCR was performed using the same PCR setup and program as July 10 except for the selection of templates and template amounts. The following templates and template amounts were used:
- #1/HS1A: 1 uL yeast sample #1. (S. Cerevisiae [1])
- #2/HS1B: 2 uL yeast sample #1. (S. Cerevisiae [1])
- #3/HS2A: 1 uL yeast sample #2. (Brettanomyces? [1])
- #4/HS2B: 2 uL yeast sample #2. (Brettanomyces?[1])
- #5/P1: 1 uL S. cerevisiae (Idun tørrgjær. Template prepared 5 july stored in freezer)
- #6/P2: 2 uL S. cerevisiae (Idun tørrgjær. Template prepared 5 july stored in freezer)
- #7/NT: Reaction mix and primers only, no template.
The PCR samples were moved to freezer after completion of the PCR run.
[1]: Liquid cultures provided by Heikki. To prepare template, 50 uL liquid culture was incubated at 98C for 10 min in OpenPCR.